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| The present invention relates to the direct screening of hybridoma supernatants or other potential sources of catalysts for catalytic activity, and more particularly to a method for screening or detection of non-enzyme catalytic polypeptides and proteins, including catalytic antibodies. Catalytic antibodies are elicited against a hapten, typically, a stable synthetic analog of the transition state (TS) of the catalyzed reaction. The repertoire resulting after immunization is immortalized as hybridomas, which are then screened to select those clones producing monoclonal antibodies that bind the hapten. The initial screening of the hybridomas is by binding assays of the antibody to the hapten. Those hybridomas are then propagated and checked for catalytic activity. This conventional approach does not identify antibodies that bind weakly to the hapten, although they may have the desired catalytic activity. In addition, this approach is time consuming and wasteful of materials. The current invention provides a more effective mean of screening hybridomas. In this method a preparation containing the potential catalyst is contacted with the substrate S immobilized to a support and the immobilized product P obtained is detected. Detecting catalytic activity directly in the supernatent is more precise and efficient than the current methods.present relates screening hybridoma supernatants potential sources catalysts catalytic activity detection non-enzyme polypeptides proteins antibodies |
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